Activated Ha-ras can cooperate with defective simian virus 40 in the transformation of nonestablished rat embryo fibroblasts
نویسندگان
چکیده
منابع مشابه
Activated Ha-ras can cooperate with defective simian virus 40 in the transformation of nonestablished rat embryo fibroblasts.
We studied the ability of activated Ha-ras to cooperate with simian virus 40 (SV40) in the transformation of nonestablished rat embryo fibroblasts. Cotransfection with Ha-ras greatly accelerated the rate of focus induction by wild-type SV40. Moreover, a series of transformation-defective SV40 mutants could be partially complemented by Ha-ras. This was true not only for mutants retaining an inta...
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The transformation of primary rat cells into established cell lines by eimian virus 40 has been monitared using the different restrictive assays of colony formation in sparse culture, dense colony or focus formation on a confluent cell sheet, and colony formation in semisolid medium. Primary embryonic rat cell cultures are considerably less susceptible to infection and subsequent transformation...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1987
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.61.8.2648-2654.1987